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| Registros recuperados: 64 | |
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| RAHMY,T. R.. |
| The effect of intramuscular (IM) injection of a sub-lethal dose of Naja haje cobra venom (0.015 mug/gm body weight) on the ultrastructure of renal cortical tissues of rabbits was studied at different time intervals after envenomation. Succinic dehydrogenase (SDH) enzyme activity was also detected in the renal cortical tubules. Three hours after venom injection, slight changes were seen in visceral cells and glomerular endothelia. The cortical tubular epithelia revealed an increase of lysosomal structures, cytoplasmic vacuolization, and nuclear irregularity. Severe ultrastructural changes were recorded 6 hours after envenomation, including hypertrophied parietal cells, blebbed visceral epithelial cells, and foot process disorganization. Dilated glomerular... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Snake venom; Nephrotoxicity; Kidney; Ultrastructure. |
| Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79302001000100007 |
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| LEITE,C.V.S.; NARESSE,L.E.; ARANTES,H.L.; LOPES,A.F.; THOMAZINI-SANTOS,I.A.; GIANNINI,M.J.S; MERCADANTE,M.C.; BARRAVIERA,B.; KOBAYASI,S.. |
| The objective of this study was to evaluate the efficacy of fibrin adhesive made up of snake venom and bubaline fibrinogen by rat colon anastomosis. Eighty rats were randomly assigned into 2 experimental groups: GI control (anastomosis with extramucous interrupted suture) and GII (repair suture + fibrin glue). The animals were studied at the following 4 times: T0 preoperative, T1 7th day postoperative, T2 14th day postoperative, and T3 21 st day postoperative. The macroscopic characteristics of the intestinal segment open and closed anastomosis and the bursting strength of the anastomosed segments were observed at each of the above times. The results showed that the anastomosed segments coapted and there was no difference in the bursting strength... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Fibrin glue; Experimental healing; Bursting strength; Experimental anastomosis; Snake venom; Colon anastomosis. |
| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79302000000200004 |
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| BARRAVIERA,B.; LOMONTE,B.; TARKOWSKI,A.; HANSON,L. Å.; MEIRA,D.A.. |
| Thirty-one patients bitten by venomous snakes in Botucatu area (State of São Paulo - Brazil), sixteen by Bothrops spp. and fifteen by Crotalus durissus terrificus, were studied. The group comprised twenty-nine males and two females, ranging from fourteen to sixty-three years of age (mean 33 ± 15). Leukocytosis with neutrophilia and lymphopenia, increase of mucoproteins and C-reactive protein, decrease of total serum protein and albumin, were observed on the first day after the accident. In addition, increased serum levels of the cytokines IL-6 and IL-8, but not of IL-1<FONT FACE="Symbol">b</FONT> and TNF-<FONT FACE="Symbol">a</FONT>, were observed. The alterations were generally more intense in patients bitten by Crotalus durissus... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Snake venom; Acute-phase reactions; Cytokines; C-reactive protein; Inflammation. |
| Ano: 1995 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79301995000100003 |
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| THOMAZINI-SANTOS,I. A.; BARRAVIERA,S. R. C. S.; MENDES-GIANNINI,M. J. S.; BARRAVIERA,B.. |
| The authors have performed a literature review of surgical adhesives, such as cyanoacrylate, collagen gelatin, and fibrin glue. They have included different types of commercial and non-commercial fibrin sealants and have reported on the different components in these adhesives, such as fibrinogen, cryoprecipitate, bovine thrombin, and thrombin-like fraction of snake venom. |
| Tipo: Info:eu-repo/semantics/review |
Palavras-chave: Fibrin glue; Surgical adhesives; Collagen gelatin; Cyanoacrylates; Fibrinogen; Cryoprecipitate; Bovine thrombin; Thrombin-like fraction; Snake venom. |
| Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79302001000200002 |
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| MADY,E. A.. |
| The search for snake venom antitumor efficacy has attracted the interest of scientists since the beginning of last century. Snake venom possesses a wide spectrum of biological activities. In this study, we evaluated the effect of Echis coloratus crude venom on the evolution of Ehrlich ascites carcinoma cells (EAC). Normal and EAC-bearing mice were treated with 0.2 mg/kg body weight of crude venom. Crude venom was seen to suppress tumor growth by significantly decreasing EAC cell count and cell viability (p<0.01). There was also a significant increase in survival time of the venom-treated tumor-bearing mice (52.3%, p<0.05) in comparison to the non-treated tumor-bearing counterparts. The study of venom effect and/or tumor inoculation on some important... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Snake venom; Echis coloratus; Ehrlich ascites carcinoma; Tumor growth; Biochemistry. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79302002000200008 |
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| Jamunaa,A; Vejayan,J; Halijah,I; Sharifah,SH; Ambu,S. |
| Cytotoxicity of venoms from eleven medically important snakes found in Southeast Asia (Naja kaouthia, Naja siamensis, Naja sumatrana, Ophiophagus hannah, Bungarus candidus, Bungarus fasciatus, Enhydrina schistosa, Calloselasma rhodostoma, Trimeresurus purpureomaculatus and Tropidolaemus sumatranus) was determined, based on the MTS cytotoxicity assay, which determines the survival of viable cells in monolayer MDCK and Vero cell cultures upon exposure to the snake venoms. Snake venom toxicity was expressed as the venom dose that killed 50% of the cells (CTC50) under the assay conditions. Venoms of C. rhodostoma (2.6 µg/mL, 1.4 µg/mL) and O. hannah were the most cytotoxic (3.8 µg/mL, 1.7 µg/mL) whereas N. siamensis venom showed the least cytotoxicity (51.9... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cytotoxicity; Snake venom; Vipers; Elapids; Kraits. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992012000200004 |
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| Buchaim,Daniela Vieira; Cassaro,Claudia Vilalva; Shindo,João Vitor Tadashi Cosin; Coletta,Bruna Botteon Della; Pomini,Karina Torres; Rosso,Marcelie Priscila de Oliveira; Campos,Leila Maria Guissoni; Ferreira Jr,Rui Seabra; Barraviera,Benedito; Buchaim,Rogério Leone. |
| Abstract Fibrin biopolymers, previously referred as “fibrin glue” or “fibrin sealants”, are natural biomaterials with diverse applications on health. They have hemostatic, adhesive, sealant, scaffold and drug delivery properties and have become widely used in medical and dental procedures. Historically, these biomaterials are produced from human fibrinogen and human or animal thrombin, and the possibility of transmission of infectious diseases by human blood is not ruled out. In the 1990s, to overcome this problem, a new heterologous biomaterial composed of a thrombin-like enzyme purified from Crotalus durissus terrificus venom and a cryoprecipitate rich in fibrinogen extracted from buffaloes Bubalus bubalis blood has been proposed. Therefore, a systematic... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Fibrin sealant; Snake venom; Thrombin-like enzyme; Cryoprecipitate coagulum. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992019000100207 |
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| Cedro,Rafhaella C. A.; Menaldo,Danilo L.; Costa,Tássia R.; Zoccal,Karina F.; Sartim,Marco A.; Santos-Filho,Norival A.; Faccioli,Lúcia H.; Sampaio,Suely V.. |
| Abstract Background: Snake venom phospholipases A2 (PLA2s) have been reported to induce myotoxic, neurotoxic, hemolytic, edematogenic, cytotoxic and proinflammatory effects. This work aimed at the isolation and functional characterization of a PLA2 isolated from Bothrops jararaca venom, named BJ-PLA2-I. Methods and Results: For its purification, three consecutive chromatographic steps were used (Sephacryl S-200, Source 15Q and Mono Q 5/50 GL). BJ-PLA2-I showed acidic characteristics, with pI~4.4 and molecular mass of 14. 2 kDa. Sequencing resulted in 60 amino acid residues that showed high similarity to other Bothrops PLA2s, including 100% identity with BJ-PLA2, an Asp49 PLA2 previously isolated from B. jararaca venom. Being an Asp49 PLA2, BJ-PLA2-I... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Snake venom; Bothrops jararaca; Phospholipase A2; Inflammation; Cytotoxicity. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992018000100324 |
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| Ferreira Jr,Rui Seabra; Barros,Luciana Curtolo de; Abbade,Luciana Patrícia Fernandes; Barraviera,Silvia Regina Catharino Sartori; Silvares,Maria Regina Cavariani; Pontes,Leticia Gomes de; Santos,Lucilene Delazari dos; Barraviera,Benedito. |
| Abstract Hemostatic and adhesive agents date back to World War II, when homologous fibrin sealant came onto scene. Considering that infectious diseases can be transmitted via human blood, a new heterologous fibrin sealant was standardized in the 1990s. Its components were a serine protease (a thrombin-like enzyme) extracted from the venom of Crotalus durissus terrificus snakes and a fibrinogen-rich cryoprecipitate extracted from the blood of Bubalus bubalis buffaloes. This new bioproduct has been used as a coagulant, sealant, adhesive and recently as a candidate scaffold for mesenchymal stem cells and bone and cartilage repair. This review discusses the composition of a new heterologous fibrin sealant, and cites published articles related to its... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Fibrin sealant; Snake venom; Cryoprecipitate coagulum; Thrombin-like enzyme; Buffaloes. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992017000100204 |
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| Boldrini-França,Johara; Pinheiro-Junior,Ernesto Lopes; Arantes,Eliane Candiani. |
| ABSTRACT Background: The prevalent class of snake venom serine proteases (SVSP) in Viperidae venoms is the thrombin-like enzymes, which, similarly to human thrombin, convert fibrinogen into insoluble fibrin monomers. However, thrombin-like serine proteases differ from thrombin by being unable to activate factor XIII, thus leading to the formation of loose clots and fibrinogen consumption. We report the functional and biological characterization of a recombinant thrombin-like serine protease from Crotalus durissus collilineatus, named rCollinein-1. Methods: Heterologous expression of rCollinein-1 was performed in Pichia pastoris system according to a previously standardized protocol, with some modifications. rCollinein-1 was purified from the culture... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Snake venom; Serine proteases; Thrombin-like enzymes; Coagulation. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992019000100306 |
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| Sciani,Juliana Mozer; Pimenta,Daniel Carvalho. |
| Abstract Bradykinin-potentiating peptides (BPPs) are molecules discovered by Sergio Ferreira – who found them in the venom of Bothrops jararaca in the 1960s – that literally potentiate the action of bradykinin in vivo by, allegedly, inhibiting the angiotensin-converting enzymes. After administration, the global physiological effect of BPP is the decrease of the blood pressure. Due to this interesting effect, one of these peptides was used by David Cushman and Miguel Ondetti to develop a hypotensive drug, the widely known captopril, vastly employed on hypertension treatment. From that time on, many studies on BPPs have been conducted, basically describing new peptides and assaying their pharmacological effects, mostly in comparison to captopryl. After... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bothrops jararaca; Venom; Snake venom; Bradykinin-potentiating peptides; BPP; Modules. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992017000100208 |
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| Matias,Mariana Santos; Sousa,Bruna Barbosa de; Pereira,Déborah Fernanda da Cunha; Dias,Edigar Henrique Vaz; Mamede,Carla Cristine Neves; Queiroz,Mayara Ribeiro de; Silva,Anielle Christine Almeida; Dantas,Noelio Oliveira; Soares,Andreimar Martins; Costa,Júnia de Oliveira; Oliveira,Fábio de. |
| Abstract Background: Snake venoms are a complex mixture of proteins, organic and inorganic compounds. Some of these proteins, enzymatic or non-enzymatic ones, are able to interact with platelet receptors, causing hemostatic disorders. The possible therapeutic potential of toxins with antiplatelet properties may arouse interest in the pharmacological areas. The present study aimed to purify and characterize an antiplatelet DC protein from Bothrops alternatus snake venom. Methods: The protein, called BaltDC (DC protein from B. alternatus snake venom), was purified by a combination of ion-exchange chromatography on DEAE-Sephacel column and gel filtration on Sephadex G-75. The molecular mass was estimated by polyacrylamide gel electrophoresis in the presence... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Snake venom; Bothrops alternatus; DC protein; Platelet aggregation. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992017000100324 |
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| Meléndez-Martínez,David; Muñoz,Juan Manuel; Barraza-Garza,Guillermo; Cruz-Peréz,Martha Sandra; Gatica-Colima,Ana; Alvarez-Parrilla,Emilio; Plenge-Tellechea,Luis Fernando. |
| Abstract Background Globally, snake envenomation is a well-known cause of death and morbidity. In many cases of snakebite, myonecrosis, dermonecrosis, hemorrhage and neurotoxicity are present. Some of these symptoms may be provoked by the envenomation itself, but others are secondary effects of the produced oxidative stress that enhances the damage produced by the venom toxins. The only oxidative stress effect known in blood is the change in oxidation number of Fe (from ferrous to ferric) in hemoglobin, generating methemoglobin but not in other macromolecules. Currently, the effects of the overproduction of methemoglobin derived from snake venom are not extensively recorded. Therefore, the present study aims to describe the oxidative stress induced by... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Attenuated total reflectance-Fourier transform infrared spectroscopy; Crotalus molossus nigrescens; Venom; Snake venom; Methemoglobin; Oxidative stress; Oxyhemoglobin. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992017000100311 |
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| El-Aziz,Tarek Mohamed Abd; Khoury,Sawsan Al; Jaquillard,Lucie; Triquigneaux,Mathilde; Martinez,Guillaume; Bourgoin-Voillard,Sandrine; Sève,Michel; Arnoult,Christophe; Beroud,Rémy; Waard,Michel De. |
| Abstract Background Sperm contains a wealth of cell surface receptors and ion channels that are required for most of its basic functions such as motility and acrosome reaction. Conversely, animal venoms are enriched in bioactive compounds that primarily target those ion channels and cell surface receptors. We hypothesized, therefore, that animal venoms should be rich enough in sperm-modulating compounds for a drug discovery program. Our objective was to demonstrate this fact by using a sperm-based phenotypic screening to identify positive modulators from the venom of Walterinnesia aegyptia. Methods Herein, as proof of concept that venoms contain interesting compounds for sperm physiology, we fractionated Walterinnesia aegyptia snake venom by RP-HPLC and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Snake venom; Walterinnesia aegyptia; Bioactive compounds; Fertility; Sperm motility; Venomics; Tandem mass spectrometry; De novo sequencing; Edman degradation. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992018000100301 |
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| Castanheira,Letícia Eulalio; Rodrigues,Renata Santos; Boldrini-França,Johara; Fonseca,Fernando PP; Henrique-Silva,Flávio; Homsi-Brandeburgo,Maria I; Rodrigues,Veridiana M. |
| Background Hyaluronate is one of the major components of extracellular matrix from vertebrates whose breakdown is catalyzed by the enzyme hyaluronidase. These enzymes are widely described in snake venoms, in which they facilitate the spreading of the main toxins in the victim’s body during the envenoming. Snake venoms also present some variants (hyaluronidases-like substances) that are probably originated by alternative splicing, even though their relevance in envenomation is still under investigation. Hyaluronidases-like proteins have not yet been purified from any snake venom, but the cDNA that encodes these toxins was already identified in snake venom glands by transcriptomic analysis. Herein, we report the cloning and in silicoanalysis of the first... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Alternative splicing; Hyaluronidase-like; Snake venom. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992014000200330 |
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| Tasima,Lídia J.; Serino-Silva,Caroline; Hatakeyama,Daniela M.; Nishiduka,Erika S.; Tashima,Alexandre K.; Sant’Anna,Sávio S.; Grego,Kathleen F.; Morais-Zani,Karen de; Tanaka-Azevedo,Anita M.. |
| Abstract Background: Crotalus durissus is considered one of the most important species of venomous snakes in Brazil, due to the high mortality of its snakebites. The venom of Crotalus durissus contains four main toxins: crotoxin, convulxin, gyroxin and crotamine. Venoms can vary in their crotamine content, being crotamine-negative or -positive. This heterogeneity is of great importance for producing antivenom, due to their different mechanisms of action. The possibility that antivenom produced by Butantan Institute might have a different immunorecognition capacity between crotamine-negative and crotamine-positive C. durissus venoms instigated us to investigate the differences between these two venom groups. Methods: The presence of crotamine was analyzed... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Rattlesnake; Snake venom; Toxins; Venom variation; Antivenom. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992020000100307 |
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| Registros recuperados: 64 | |
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